Journal: Biosensors

Article Title: Point-of-Care Diagnostics for Farm Animal Diseases: From Biosensors to Integrated Lab-on-Chip Devices

doi: 10.3390/bios12070455

Figure Lengend Snippet: Available electrochemical and optical biosensors for the detection of mastitis and animal diseases. The available literature for the remaining transducing options is included in the main text.

Article Snippet: Swine-origin influenza A H1N1 virus , Anti-H1 antibody (ProSci, Poway, CA, USA) , SPR chip (BK7 glass slide coated with a laminated Ag/Au 37/8 nm, metal layer), paired-surface plasma-wave biosensor , Surface plasmon resonance (SPR) , Mimic solution (human mucosa in PBS) , Theoretical LOD of 30 PFU/mL, 1.8 × 10 2 PFU/mL. Detection in 20 min , [ ] .

Techniques: Impedance Spectroscopy, Bioprocessing, Produced, Virus, Magnetic Beads, Affinity Chromatography, Purification, Avidin-Biotin Assay, Recombinant, Irradiation, Biomarker Discovery, Electrochemiluminescence, Bacteria, Clinical Proteomics, SPR Assay, Circular Dichroism, Labeling, Fluorescence, Spectrophotometry, Förster Resonance Energy Transfer, Modification, Membrane, Imaging, Refractive Index

Journal: Viruses

Article Title: Receptor Binding-Induced Conformational Changes in Herpes Simplex Virus Glycoprotein D Permit Interaction with the gH/gL Complex to Activate Fusion

doi: 10.3390/v15040895

Figure Lengend Snippet: Characterization of wt and cys2 proteins. ( a ) Diagram of the Biacore 3000 protocol for analyzing gH/gL binding to gD, wt or cys2. ( b ) Soluble wt 306t and cys2 306t proteins were attached to the biosensor chip via 1D3 Mab. Soluble gH/gL was injected across the chip surface. Only the gH/gL binding curves are shown. Black curve, binding of gH/gL to wt gD; brown curve, binding of gH/gL to cys2. ( c ) Diagram of the Biacore 3000 protocol for analyzing the binding of gH/gL to a gD-nectin-1 receptor complex. gD molecules were attached to the biosensor chip via 1D3 Mab. Sequential injection of nectin-1 followed by injection of soluble gH/gL. ( d ) Nectin-1 binds to both wt (dark purple) and cys2 (light purple) gD proteins. ( e ) gH/gL bound to wt gD (blue) but not to cys2 mutant gD (cyan). ( f ) Diagram of the Biacore 3000 protocol for analyzing gD–gH/gL binding in the presence of TCEP. gD molecules were attached to the biosensor chip via 1D3 Mab. Nectin-1 was pre-mixed with 1 mM TCEP and flowed over the captured gD molecules, followed by the injection of soluble gH/gL. ( g ) Nectin-1 binds to both wt (purple) and cys2 (light purple) gD proteins. ( h ) As expected, gH/gL bound to wt gD (blue). Due to the reduction of the disulfide bond, gH/gL is now able to bind cys2 mutant gD as well (cyan).

Article Snippet: To address this hypothetical model, we adapted the Biacore 3000 biosensor system (SPR) assay ( a).

Techniques: Binding Assay, Injection, Mutagenesis

Journal: Viruses

Article Title: Receptor Binding-Induced Conformational Changes in Herpes Simplex Virus Glycoprotein D Permit Interaction with the gH/gL Complex to Activate Fusion

doi: 10.3390/v15040895

Figure Lengend Snippet: Ability of gD L268N to bind gH/gL. ( a ) Diagram of the Biacore 3000 protocol for analyzing the binding of gH/gL to a gD-receptor complex. gD molecules, wt, or L268N, both as 306t truncations were attached to the biosensor chip via 1D3 Mab. Sequential injection of nectin-1 followed by injection of soluble gH/gL. ( b ) Nectin-1 binds to both wt (purple) and L268N (pink) gD proteins. ( c ) gH/gL was flowed over the gD-nectin-1 complexes. gH/gL bound to wt gD (blue) but not to L268N mutant gD (cyan). ( d ) Diagram of the SPR protocol for analyzing gH/gL binding to gD 285t , wt, or the L268N mutant. Only the gH/gL binding curves are shown. gH/L binds to wt gD (black curve). No gH/gL binding was observed for the L268N mutant (orange curve).

Article Snippet: To address this hypothetical model, we adapted the Biacore 3000 biosensor system (SPR) assay ( a).

Techniques: Binding Assay, Injection, Mutagenesis

Journal: HardwareX

Article Title: BioSamplr: An open source, low cost automated sampling system for bioreactors

doi: 10.1016/j.ohx.2021.e00177

Figure Lengend Snippet: A comparison of bioreactor autosamplers.

Article Snippet: A few commercial autosampling systems available for lab bioreactors include the BioProbe™ from Bbi-Biotech, the Omnicoll Fraction Collector and Sampler from Lambda Instruments, the Segflow™ by Flownamics, and the more general reactor sampling system, the EasySampler™ by Mettler Toledo.

Techniques: Filtration, Sampling